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Anhui Medical University human physical examination peripheral blood samples
RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the <t>peripheral</t> blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.
Human Physical Examination Peripheral Blood Samples, supplied by Anhui Medical University, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+peripheral+blood+samples/peripheral+blood+samples/pmc11970327-24-0-11
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human physical examination peripheral blood samples - by Bioz Stars, 2026-10
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1) Product Images from "RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus"

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

Journal: Cell Reports Medicine

doi: 10.1016/j.xcrm.2025.101974

RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.
Figure Legend Snippet: RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.

Techniques Used: Expressing, Staining, Western Blot


Figure Legend Snippet:

Techniques Used: Recombinant, SYBR Green Assay, Multiplex Assay, Immunohistochemical staining, Staining, Reverse Transcription, Modification, Plasmid Preparation, Software, Microscopy

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Article Snippet: Bronchopulmonary dysplasia (BPD), caused by hyperoxia exposure, is the most common complication affecting preterm infants.. The C-X-C motif chemokine ligand 17 (CXCL17) belongs to the chemokine family that plays important roles in various processes, but the function in BPD is unknown.. Elevated serum CXCL17 levels were observed in human premature infants with hyperoxia-induced lung injury, suggesting that CXCL17 might be involved in BPD.

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Article Snippet: We also appreciate the support of Professor Li Qingsheng and Dr Hong Jian from the Blood Center at the First Affiliated Hospital of Anhui Medical University for providing human peripheral blood samples.



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RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the <t>peripheral</t> blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.
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RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the <t>peripheral</t> blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.
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RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet: RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.

Article Snippet: Human physical examination peripheral blood samples , First Affiliated Hospital of Anhui Medical University , N/A.

Techniques: Expressing, Staining, Western Blot

Journal: Cell Reports Medicine

Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus

doi: 10.1016/j.xcrm.2025.101974

Figure Lengend Snippet:

Article Snippet: Human physical examination peripheral blood samples , First Affiliated Hospital of Anhui Medical University , N/A.

Techniques: Recombinant, SYBR Green Assay, Multiplex Assay, Immunohistochemical staining, Staining, Reverse Transcription, Modification, Plasmid Preparation, Software, Microscopy